What has happened to the scientific process that most of us were taught while in graduate school? The idea that experiments need to be well thought out and contain the proper positive and negative controls. The need for BOTH technical as well as biological replicates and clear well written methods sections so that other laboratories can verify and extend published work. In my opinion, one area where these ideas can often fall short is with in vitro or cell based systems for evaluating chemical effects. Three wells does not represent a true (N). Separate plates on separate days with cells starting from cryo or from isolation should be a requirement. The concepts of dose (exposure concentration) and time are just as important in vitro as they are in vivo. So why are there so many papers published that ignore all of these?