Dermal Corrosion 

Dermal Corrosion

Dermal Corrosion Test

Predict the potential of your test compound or xenobiotic to cause dermal corrosion using our Skin Corrosion Test (SCT). 

This test is performed according to the Organization for Economic Co-operation and Development (OECD) test guideline number 431 and allows for proper labelling of the test compound or xenobiotic according to United Nations Globally Harmonized System (UN GHS) Classification and Labelling of Chemicals.1

Historically, dermal corrosion was performed on rabbits using the Draize Skin Test.2  However, three dimensional human epidermis tissue equivalents have been developed and validated as replacements for the corrosion test.  These 3D tissues are dermal epithelia grown from normal human keratinocytes and are extremely similar to native human epidermis, both metabolically and morphologically, including the stratum corneum.  In addition, the apical surface is direct contact with the air (tissue is fed basally) allowing for testing of almost all types materials, such as gels, creams liquids and powders, regardless of aqueous solubility.

Assay Overview

In this assay, MatTek EpiDerm™ tissues are exposed on the apical surface to neat (undiluted) test chemical for 3 minutes and 60 minutes and the viability of the tissue is then determined via the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.  This data is then normalized to the negative control and ocular irritation is determined based on the viability using the following table:

Mean Tissue Viability In Vivo Prediction
< 50% after 3 min exposure Corrosive (Category 1)
≥ 50% after 3 min exposure and < 15% after 60 min exposure Corrosive (Category 1)
≥ 50% after 3 min exposure and ≥ 15% after 60 min exposure Non-Corrosive
Optional Sub-categorizations
Mean Tissue Viability In Vivo Prediction
< 25% after 3 min exposure Optional Sub-category 1A
≥ 25% after 3 min exposure Optional Sub-category 1B
References
    OECD (2015) OECD Guidelines for the Testing of Chemicals, Section 4. Test Guideline 439. In vitro skin corrosion: reconstructed human epidermis (RHE) test method. Organization for Economic Co-operation and Development, Paris, July 28, 2015.
    Draize J.H., Woodard G., and Calvery H.O. (1944). Methods for the Study of Irritation and Toxicity of Substances Applied Topically to the Skin and Mucous Membranes. J Pharm Exp Ther 82, 377-390.