Using rat (H4IIe) or human (HepG2) cell lines or primary hepatocytes, researchers can study their compound’s potential to cause oxidative stress in vitro. In this assay, toxicity is measured through the activation of Nrf2, signaling pathways and target genes known to be indicators of oxidative stress. In addition to measuring Nrf2 signaling pathways, the depletion of glutathione (GSH) and activation of dichlorofluorescein diacetate (DCFDA) are also measured.
Together, this panel of endpoints provides clear information of oxidative damage through metabolism and the production of reactive oxygen species.
Standard offering of the Oxidative Stress Assay includes positive and negative controls, indicatorsof Nrf2 signaling, changes in reduced and oxidized GSH (GSH/GSSG ratio), and activation of DCFDA. Each compound is evaluated at seven exposure concentrations, each in triplicate, and a single time point . Researchers also have the choice of utilizing primary hepatocytes for their study.