Screen your compounds using in vitro 3D tissue models.

IonTox can conduct a range of in vitro assays on reconstructed human tissue, to replace animal testings and make findings that are more applicable to humans. 

We can test on 3D tissue models of skin, corneal tissue, and lung airways. Our assays can predict systemic toxicity, irritation, and corrosion. All 3D testing is run in accordance with OECD guidelines. Results can be used to ensure the safety of your compound and support your applications for regulatory approval. 

Applications of 3D Tissue Testing 

3D tissue testing provides a superior alternative to testing on animal models, especially for companies developing pharmaceuticals and cosmetics. Advances in 3-dimensional tissue modeling make it possible to predict adverse effects and viability of drug candidates and new cosmeceuticals. 

Reconstructed human skin tissue, in particular, replaces skin irritation and corrosion tests historically conducted on animals. As many countries move to ban animal testing of cosmetics, it’s a viable alternative that allows you to validate the safety of your products. 

IonTox collaborates with the world’s leading biotechnology companies to source 3D tissue models. Our main suppliers are MatTek, Epithelix, Corp, and Skin Ethic. 

In addition to simple 3D tissue tests, we are also able to combine 3D tissue testing with HuDMOP®, a flexible microphysiological system that replicates interconnected organs. This allows us to determine systemic responses to a test compound and provides toxicity and metabolism data that is highly applicable to humans. 

IonTox 3D Tissue Testing Assays

These are the 3D tissue assays and panels offered by IonTox. Our scientists are able to recommend the assays or panels that will be most applicable to your company’s needs. 

Dermal Irritation

Using reconstructed human skin models, this test predicts the potential of a compound to induce reversible skin irritation. It’s conducted in compliance with OECD test guideline no. 439. It’s an affordable, ethical replacement for animal skin irritation tests. We can also conduct Sensitization Testing panels to predict the potential of a chemical to cause allergic responses in accordance with OECD guidelines no. 442C, D, or E. 

Dermal Corrosion

Using 3D dermal tissue models, this test predicts the potential of your compound to cause irreversible damage to the skin, also known as corrosion. This assay follows OECD test guideline no. 431 and can be used for the accurate labeling of your compound according to UN GHS. 

Ocular Irritation

This assay uses 3D human corneal tissue to predict the likelihood that a compound or chemical will cause eye irritation. This test follows OECD guideline no. 492 and is carried out using MatTek EpiOcular™ tissue. 

Acute Oral Toxicity Predictor

The In Vitro Acute Oral Toxicity Predictor is a method developed by IonTox to predict the median lethal dose (LD50) of a new chemical, without the need for testing on living animals.

Inhalation (Respiratory) Toxicity

Using reconstructed models of human lung airways, this test predicts the inhalation cytotoxicity risk posed by liquid, aerosol, or powder test chemicals. By connecting the lung model to a liver model, systemic toxicity can also be assessed. 

Dermal Systemic Toxicity

This assay determines dermal penetration, metabolism, and liver toxicity. By combining 3D dermal tissue models with a unique multi-organ microphysiological system, IonTox is able to predict the potential of a compound to cause systemic toxicity via dermal penetration.